Library Preparation Kits
- Home
- Library Preparation Kits
Library Preparation Kits
➧ DNA Library Preparation kit
NadPrep DNA Library Preparation Module v2 is designed for the preparation of high-quality libraries from double-stranded DNA (dsDNA) on Illumina® & MGI platforms. This A-T ligation-based kit offers a stable and efficient library preparation solution for ap plications including whole genome sequencing of 1‒500 ng DNA and hybridization capture based targeted sequencing. Comp ared to the v1 version, the upgraded kit has improved the library conversion efficiency and better tolerance for low-quality sam ples. Consequently, it can enhance library complexity while maintaining high fidelity.
➧ EZ Library Preparation kit
NadPrep EZ DNA Library Preparation Kit v2 is designed for preparation of high-quality sequencing libraries from double-stranded DNA (dsDNA) on MGI and Illumina platforms. To simplify the experimental process, multiple processes were applied in one single step, including the fragmentation, end repair and adapter ligation. This A-T ligation-based kit applies to the whole genome sequencing with DNA input ranging from 5 to 500 ng, and is compatible with hybridization capture based targeted sequencing.
➧ IGTR Multiplex Library Preparation Kit
NadPrep IGTR Multiplex PCR Library Prep Kit is based on the principle of multiplex amplification, enabling efficient enrichment of the complementary determining regions (CDR3) of seven loci for human adaptive immune antigen receptors, including IGH, IGK, IGL, TRA, TRB, TRG and TRD. Combined with high-throughput sequencing technology, this kit facilitates precise detection of B-cell and T-cell diversity and clonality. It is compatible with human DNA or cDNA initial samples from various sources and is widely applicable for immune repertoire studies, clonality analysis of lymphoid malignancies, and minimal residual disease (MRD) monitoring.
➧ Methyl Library Preparation Kit
NadPrep Methyl Library Preparation Kit v2 is designed for preparation of high-quality methylation sequencing (Methyl-Seq) libraries from double-stranded DNA (dsDNA) on both Illumina and MGI platforms. This kit is suitable for preparing Methyl-Seq libraries from 1-500 ng DNA, supporting whole-genome bisulfite sequencing (WGBS) as well as hybrid capture-based targeted methylation sequencing with effective enrichment of targeted libraries.
➧ Methyl Bisulfite Conversion Module
NadPrep DNA Methyl Bisulfite Conversion Module is a methylation conversion reagent developed for DNA samples based on the principle of bisulfite conversion. The conversion module, based on a magnetic bead purification protocol, efficiently conv- erts unmethylated cytosine to uracil within 2 hr, leaving methylated cytosine unchanged, and it can be compatible with autom- ated workstations. It can be seamlessly integrated with Nanodigmbio’s NadPrep double-strand methylation library preparation scheme and NadPrep single-strand methylation library preparation scheme, allowing flexible selection among various types of starting samples.
➧ RNA & DNA Library Co-preparation module
NadPrep RNA & DNA Library Co-Preparation Module is developed for high-throughput sequencing platforms, enabling a single process for RNA & DNA co-preparation from mixed pathogenic microbial samples. This module consist of 1st Strand Synthesis, 2 nd Strand Synthesis, Fragmentation and End Repair & A-Tailing, Adapter Ligation and Amplification. It can be used in conjunction with the NadPrep Universal Stubby Adapter (UDI) Module and NadPrep Universal Adapter (MDI) Module (for MGI) for different sequencing platforms
➧ Total RNA to DNA module
NadPrep Total RNA-To-DNA Module is a reverse transcription module developed for NGS platform. This module offers an efficient solution for the generation of dsDNA fragments from 10-200 ng of Human total RNA from cells, tissues and FFPE samples.The dsDNA output can couple with the NadPrep DNA Library Preparation kit.
➧ Universal Circularization Kit
The NadPrep Universal Circularization Kit v2 is designed for preparation of a single-stranded circular DNA (ssCirDNA) library for the MGI platform based on DNBSEQTM technology. The circularization module inside is compatible with the circularization of libraries with single index (SI) adapters and dual index (DI) adapters on MGI platform, and NadPrep Universal Stubby Adapters (UDI), and is suitable for the circularization of libraries converted by the MGIEasy Universal Library Conversion Kit (App-A), with the preparation of single-stranded circular libraries effectively streamlined.
➧ rRNA Blocking Reagent
NadPrep rRNA Blocking Reagent is a specialized processing module developed for high-throughput sequencing platforms to rapidly block rRNA in total RNA. This kit-series offer both commercial and customized solutions, supporting species-specific designs. It is suitable for total RNA with initial input amounts ranging from 50-500 ng, sourced from cells, tissues, and FFPE samples. If you require a customized design for a specific species, please contact us at (support@njnad.com) for professional, personal advice.